Journal: Materials Today Bio

Article Title: A novel fertility-sparing nanocomposite for endometriosis treatment via oxidative stress and inflammation alleviation

doi: 10.1016/j.mtbio.2025.102041

Figure Lengend Snippet: The impact of nanocomposites on macrophage polarization and the expression of related genes (A) M0 marker expression in THP-1 cells was assessed following induction by PMA, PMA + IFN-γ+ LPS, PMA + IFN-γ+ LPS + NCs, PMA + IL-4+ IL-13, and PMA + IL-4+ IL-13+NCs. CD68 expression was detected using flow cytometry, while gene levels of CD68 and CD14 were assessed by qRT-PCR. (B) M1 marker expression in THP-1 cells was assessed following induction by PMA, PMA + IL-4+ IL-13, PMA + IFN-γ+ LPS, and PMA + IFN-γ+ LPS + NCs. CD86 expression was detected using flow cytometry, and gene levels of TNF-α, IL-6, CXCL10, CD80, and CD86 were assessed by qRT-PCR. (C) M2 marker expression in THP-1 cells was assessed following induction by PMA, PMA + IFN-γ+ LPS, PMA + IL-4+ IL-13, and PMA + IL-4+ IL-13+NCs. CD206 expression was detected using flow cytometry, and gene levels of CCL22, CD206, and TGF-β were assessed by qRT-PCR. Not significant (ns): p > 0.05, ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗: p < 0.001.

Article Snippet: For the induction of M2 macrophage, the culture medium was refreshed with fresh medium containing 100 ng/ml PMA, 20 ng/ml IL-4 (MCE), and 20 ng/ml IL-13 (MCE), and after 72 h, M2 macrophages were obtained.

Techniques: Expressing, Marker, Flow Cytometry, Quantitative RT-PCR

Journal: Frontiers in Immunology

Article Title: Blocking LAIR1 signaling in immune cells inhibits tumor development

doi: 10.3389/fimmu.2022.996026

Figure Lengend Snippet: Inhibition of LAIR1 enhances dendritic cell differentiation and dendritic cell- induced T cell proliferation in MLR assay. (A) CD14 + monocytes isolated from fresh human PBMCs were cultured with GM-CSF and IL-4 for 2 days. The expression of CD11c on CD14 negative population and CD86 were detected. (B, C) Matured DCs were mixed with allogenic T cells in the MLR assay. Surface CD25 (B) and CFSE based cell proliferation were determined by flow cytometry at 72 hours (C). As Statistical Analysis in Methods stated: * indicates p < 0.05; ** indicates p < 0.01, *** indicates p < 0.001.

Article Snippet: 1x10 5 CD14 + cells were cultured in RPMI 1640 medium (R8758-500ml, Sigma) supplemented with 10% fetal bovine serum (F0926, Sigma), 2 mM L-alanine-L-glutamine (SH3003402, Fisher), 50 ng/ml GM-CSF (300-03, PeproTech) and 25 ng/ml human IL-4 (11846-HNAE-1, Sino Biological) per well of a 96-well plate for 6 days; fresh medium was replaced every 3 days.

Techniques: Inhibition, Cell Differentiation, Mlr Assay, Isolation, Cell Culture, Expressing, Flow Cytometry

Journal: iScience

Article Title: A double-edged sword role of IFN-γ-producing iNKT cells in sepsis: Persistent suppression of Treg cell formation in an Nr4a1-dependent manner

doi: 10.1016/j.isci.2024.111462

Figure Lengend Snippet:

Article Snippet: Mouse IL-4 Recombinant Protein , MedChemExpress , Cat# HY-P70653.

Techniques: Control, Virus, Recombinant, Staining, Activation Assay, Cell Isolation, Software